OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice

Expression of human cytokines dramatically improves reconstitution of specific human-blood lineage cells in humanized mice.

Adoptive transfer of human hematopoietic stem cells (HSCs) into mice lacking T, B and natural killer (NK) cells leads to development of human-blood lineage cells in the recipient mice (humanized mice). Although human B cell reconstitution is robust and T cell reconstitution is reasonable in the recipient mice, reconstitution of NK cells and myeloid cells is generally poor or undetectable. Here,...

Engineering Humanized Mice for Improved Hematopoietic Reconstitution Running Title: Improving Humanized Mice

OMIP-020: phenotypic characterization of human γδ T-cells by multicolor flow cytometry.

THIS panel was composed and optimized to investigate the differentiation stages of human cd T-cells in cryopreserved peripheral blood mononuclear cells (PBMC) from healthy individuals (Tables 1 and 2). As the majority of pan-cd T-cell antibodies available commercially proved to be inappropriate for detecting all cd T-cell populations in combination with other markers, this panel provides an ess...

Modeling Human Leukemia Immunotherapy in Humanized Mice

The currently available human tumor xenograft models permit modeling of human cancers in vivo, but in immunocompromised hosts. Here we report a humanized mouse (hu-mouse) model made by transplantation of human fetal thymic tissue plus hematopoietic stem cells transduced with a leukemia-associated fusion gene MLL-AF9. In addition to normal human lymphohematopoietic reconstitution as seen in non-...

application and construction of carbon paste modified electrodes developed for determination of metal ions in some real samples

ساخت الکترودهاِی اصلاح شده ِیکِی از چالشهاِی همِیشگِی در دانش شیمِی بوِیژه شیمِی تجزیه مِی باشد ،که با در نظر گرفتن سادگِی ساخت، کاربردی بودن و ارزان بودن روش مِی توان به باارزش بودن چنِین سنسورهاِی پِی برد.آنچه که در ادامه آورده شده به ساخت و کاربرد الکترودهاِی اصلاح شده با استفاده از نانو ذرات در اندازه گِیرِی ولتامترِی آهن وکادمِیم اشاره دارد. کار اول اختصاص دارد به ساخت الکترود خمِیر کربن اصلاح شده با لِیگاند داِ...